1SOA: Human Dj-1 With Sulfinic Acid

Citation:
Abstract
Loss-of-function DJ-1 mutations can cause early-onset Parkinson's disease. The function of DJ-1 is unknown, but an acidic isoform accumulates after oxidative stress, leading to the suggestion that DJ-1 is protective under these conditions. We addressed whether this represents a posttranslational modification at cysteine residues by systematically mutating cysteine residues in human DJ-1. WT or C53A DJ-1 was readily oxidized in cultured cells, generating a pI 5.8 isoform, but an artificial C106A mutant was not. We observed a cysteine-sulfinic acid at C106 in crystalline DJ-1 but no modification of C53 or C46. Oxidation of DJ-1 was promoted by the crystallization procedure. In addition, oxidation-induced mitochondrial relocalization of DJ-1 and protection against cell death were abrogated in C106A but not C53A or C46A. We suggest that DJ-1 protects against neuronal death, and that this is signaled by acidification of the key cysteine residue, C106.
PDB ID: 1SOADownload
MMDB ID: 28267
PDB Deposition Date: 2004/3/13
Updated in MMDB: 2017/11
Experimental Method:
x-ray diffraction
Resolution: 1.2  Å
Source Organism:
Similar Structures:
Biological Unit for 1SOA: dimeric; determined by author and by software (PISA,PQS)
Molecular Components in 1SOA
Label Count Molecule
Proteins (2 molecules)
2
RNA-binding Protein Regulatory Subunit; Oncogene DJ1(Gene symbol: PARK7)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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