1SL1: Binary 5' Complex Of T7 Dna Polymerase With A Dna Primer/template Containing A Cis-syn Thymine Dimer On The Template

Ultraviolet-induced DNA damage poses a lethal block to replication. To understand the structural basis for this, we determined crystal structures of a replicative DNA polymerase from bacteriophage T7 in complex with nucleotide substrates and a DNA template containing a cis-syn cyclobutane pyrimidine dimer (CPD). When the 3' thymine is the templating base, the CPD is rotated out of the polymerase active site and the fingers subdomain adopts an open orientation. When the 5' thymine is the templating base, the CPD lies within the polymerase active site where it base-pairs with the incoming nucleotide and the 3' base of the primer, while the fingers are in a closed conformation. These structures reveal the basis for the strong block of DNA replication that is caused by this photolesion.
PDB ID: 1SL1Download
MMDB ID: 51350
PDB Deposition Date: 2004/3/5
Updated in MMDB: 2007/10
Experimental Method:
x-ray diffraction
Resolution: 2.2  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 1SL1: tetrameric; determined by author
Molecular Components in 1SL1
Label Count Molecule
Proteins (2 molecules)
DNA Polymerase(Gene symbol: T7p29)
Molecule annotation
Thioredoxin 1(Gene symbol: trxA)
Molecule annotation
Nucleotides(2 molecules)
5'- D(*cp*gp*ap*ap*ap*ap*cp*gp*ap*cp*gp*gp*cp*cp*ap*gp*tp*gp*cp *cp*tp*(2da))-3'
Molecule annotation
5'-d(*cp*cp*c*(ttd) P*ap*gp*gp*cp*ap*cp*tp*gp*gp*cp*cp*gp*tp*cp*gp*tp*tp*tp*tp* Cp*g)-3'
Molecule annotation
Chemical (1 molecule)
* Click molecule labels to explore molecular sequence information.

Citing MMDB