1SJF: Crystal Structure Of The Hepatitis Delta Virus Gemonic Ribozyme Precursor, With C75u Mutaion, In Cobalt Hexammine Solution

Citation:
Abstract
Ribozymes enhance chemical reaction rates using many of the same catalytic strategies as protein enzymes. In the hepatitis delta virus (HDV) ribozyme, site-specific self-cleavage of the viral RNA phosphodiester backbone requires both divalent cations and a cytidine nucleotide. General acid-base catalysis, substrate destabilization and global and local conformational changes have all been proposed to contribute to the ribozyme catalytic mechanism. Here we report ten crystal structures of the HDV ribozyme in its pre-cleaved state, showing that cytidine is positioned to activate the 2'-OH nucleophile in the precursor structure. This observation supports its proposed role as a general base in the reaction mechanism. Comparison of crystal structures of the ribozyme in the pre- and post-cleavage states reveals a significant conformational change in the RNA after cleavage and that a catalytically critical divalent metal ion from the active site is ejected. The HDV ribozyme has remarkable chemical similarity to protein ribonucleases and to zymogens for which conformational dynamics are integral to biological activity. This finding implies that RNA structural rearrangements control the reactivity of ribozymes and ribonucleoprotein enzymes.
PDB ID: 1SJFDownload
MMDB ID: 27843
PDB Deposition Date: 2004/3/3
Updated in MMDB: 2012/11
Experimental Method:
x-ray diffraction
Resolution: 2.75  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 1SJF: dimeric; determined by author
Molecular Components in 1SJF
Label Count Molecule
Protein (1 molecule)
1
U1 Small Nuclear Ribonucleoprotein a(Gene symbol: SNRPA)
Molecule annotation
Nucleotide(1 molecule)
1
Hepatitis Delta Virus Ribozyme
Molecule annotation
Chemicals (2 molecules)
1
2
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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