1SIZ: Crystal Structure Of The [fe3s4]-Ferredoxin From The Hyperthermophilic Archaeon Pyrococcus Furiosus

Citation:
Abstract
The structure of [Fe(3)S(4)]-ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus has been determined to 1.5 A resolution from a crystal belonging to space group P2(1) with two molecules in the asymmetric unit. The structure has been solved with molecular replacement by use of the ferredoxin from Thermotoga maritima. The fold is similar to that of related monocluster ferredoxins and contains two double-stranded antiparallel beta-sheets and two alpha-helices. The hydrophobic interaction between Trp2 and Tyr46 is confirmed, linking the C-terminus to the longer alpha-helix. The structure contains a double-conformation disulfide bond existing in a left-handed and a right-handed spiral conformation. The crystal packing reveals a beta-sheet interaction, which supports the suggestion that P. furiosus ferredoxin is a functional dimer. The extraordinary thermostability of P. furiosus ferredoxin is further discussed.
PDB ID: 1SIZDownload
MMDB ID: 27835
PDB Deposition Date: 2004/3/2
Updated in MMDB: 2012/11
Experimental Method:
x-ray diffraction
Resolution: 2.25  Å
Source Organism:
Similar Structures:
Biological Unit for 1SIZ: dimeric; determined by author
Molecular Components in 1SIZ
Label Count Molecule
Proteins (2 molecules)
2
Ferredoxin(Gene symbol: PF_RS09645)
Molecule annotation
Chemicals (6 molecules)
1
4
2
2
* Click molecule labels to explore molecular sequence information.

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