1SIG: CRYSTAL STRUCTURE OF A SIGMA70 SUBUNIT FRAGMENT FROM ESCHERICHIA COLI RNA POLYMERASE

Citation:
Abstract
The 2.6 A crystal structure of a fragment of the sigma 70 promoter specificity subunit of E. coli RNA polymerase is described. Residues involved in core RNA polymerase binding lie on one face of the structure. On the opposite face, aligned along one helix, are exposed residues that interact with the -10 consensus promoter element (the Pribnow box), including four aromatic residues involved in promoter melting. The structure suggests one way in which DNA interactions may be inhibited in the absence of RNA polymerase and provides a framework for the interpretation of a large number of genetic and biochemical analyses.
PDB ID: 1SIGDownload
MMDB ID: 57370
PDB Deposition Date: 1997/2/18
Updated in MMDB: 2012/11
Experimental Method:
x-ray diffraction
Resolution: 2.6  Å
Source Organism:
Similar Structures:
Biological Unit for 1SIG: monomeric; determined by author
Molecular Components in 1SIG
Label Count Molecule
Protein (1 molecule)
1
RNA Polymerase Primary Sigma Factor(Gene symbol: rpoD)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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