1SHZ: Crystal Structure Of The P115rhogef Rgrgs Domain In A Complex With Galpha(13):galpha(i1) Chimera

p115RhoGEF, a guanine nucleotide exchange factor (GEF) for Rho GTPase, is also a GTPase-activating protein (GAP) for G12 and G13 heterotrimeric Galpha subunits. The GAP function of p115RhoGEF resides within the N-terminal region of p115RhoGEF (the rgRGS domain), which includes a module that is structurally similar to RGS (regulators of G-protein signaling) domains. We present here the crystal structure of the rgRGS domain of p115RhoGEF in complex with a chimera of Galpha13 and Galphai1. Two distinct surfaces of rgRGS interact with Galpha. The N-terminal betaN-alphaN hairpin of rgRGS, rather than its RGS module, forms intimate contacts with the catalytic site of Galpha. The interface between the RGS module of rgRGS and Galpha is similar to that of a Galpha-effector complex, suggesting a role for the rgRGS domain in the stimulation of the GEF activity of p115RhoGEF by Galpha13.
PDB ID: 1SHZDownload
MMDB ID: 31233
PDB Deposition Date: 2004/2/26
Updated in MMDB: 2007/10
Experimental Method:
x-ray diffraction
Resolution: 2.85  Å
Source Organism:
Homo sapiens
Similar Structures:
Biological Unit for 1SHZ: dimeric; determined by author and by software (PISA)
Molecular Components in 1SHZ
Label Count Molecule
Proteins (2 molecules)
Guanine Nucleotide-binding Protein Galpha(13):galpha(i1) Chimera
Molecule annotation
RHO Guanine Nucleotide Exchange Factor 1(Gene symbol: ARHGEF1)
Molecule annotation
Chemicals (3 molecules)
* Click molecule labels to explore molecular sequence information.

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