1RWZ: Crystal Structure Of Proliferating Cell Nuclear Antigen (Pcna) From A. Fulgidus

Flap EndoNuclease-1 (FEN-1) and the processivity factor proliferating cell nuclear antigen (PCNA) are central to DNA replication and repair. To clarify the molecular basis of FEN-1 specificity and PCNA activation, we report here structures of FEN-1:DNA and PCNA:FEN-1-peptide complexes, along with fluorescence resonance energy transfer (FRET) and mutational results. FEN-1 binds the unpaired 3' DNA end (3' flap), opens and kinks the DNA, and promotes conformational closing of a flexible helical clamp to facilitate 5' cleavage specificity. Ordering of unstructured C-terminal regions in FEN-1 and PCNA creates an intermolecular beta sheet interface that directly links adjacent PCNA and DNA binding regions of FEN-1 and suggests how PCNA stimulates FEN-1 activity. The DNA and protein conformational changes, composite complex structures, FRET, and mutational results support enzyme-PCNA alignments and a kinked DNA pivot point that appear suitable to coordinate rotary handoffs of kinked DNA intermediates among enzymes localized by the three PCNA binding sites.
PDB ID: 1RWZDownload
MMDB ID: 26215
PDB Deposition Date: 2003/12/17
Updated in MMDB: 2007/11
Experimental Method:
x-ray diffraction
Resolution: 1.8  Å
Source Organism:
Similar Structures:
Biological Unit for 1RWZ: trimeric; determined by author
Molecular Components in 1RWZ
Label Count Molecule
Proteins (3 molecules)
DNA Polymerase Sliding Clamp(Gene symbol: AF_RS01715)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB