1RUL: Crystal Structure (d) Of U.v.-irradiated Cationic Cyclization Antibody 4c6 Fab At Ph 5.6 With A Data Set Collected At Ssrl Beamline 11-1

Citation:
Abstract
Antibodies can catalyze the generation of hydrogen peroxide (H2O2) from singlet dioxygen (1O2*) and water via the postulated intermediacy of dihydrogen trioxide (H2O3) and other trioxygen species. Nine different crystal structures were determined to elucidate the chemical consequences to the antibody molecule itself of exposure to such reactive intermediates and to provide insights into the location on the antibody where these species could be generated. Herein, we report structural evidence for modifications of two specific antibody residues within the interfacial region of the variable and constant domains of different murine antibody antigen-binding fragments (Fabs) by reactive species generated during the antibody-catalyzed water oxidation process. Crystal structure analyses of murine Fabs 4C6 and 13G5 after UV-irradiation revealed complex oxidative modifications to tryptophan L163 and, in 4C6, hydroxylation of the Cgamma of glutamine H6. These discrete modifications of specific residues add further support for the "active site" of the water-oxidation pathway being located within the interfacial region of the constant and variable domains and highlight the general resistance of the antibody molecule to oxidation by reactive oxygen species generated during the water-oxidation process.
PDB ID: 1RULDownload
MMDB ID: 26576
PDB Deposition Date: 2003/12/11
Updated in MMDB: 2007/10
Experimental Method:
x-ray diffraction
Resolution: 1.88  Å
Source Organism:
Similar Structures:
Biological Unit for 1RUL: dimeric; determined by author and by software (PISA)
Molecular Components in 1RUL
Label Count Molecule
Proteins (2 molecules)
1
Immunoglobulin Igg2a, Light Chain
Molecule annotation
1
Immunoglobulin Igg2a, Heavy Chain(Gene symbol: Ighg2a)
Molecule annotation
Chemicals (6 molecules)
1
1
2
4
3
1
* Click molecule labels to explore molecular sequence information.

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