1RED: Endo-1,4-beta-xylanase Ii Complex With 4,5-epoxypentyl-beta- D-xyloside

The three-dimensional structures of endo-1,4-xylanase II (XYNII) from Trichoderma reesei complexed with 4,5-epoxypentyl beta-D-xyloside (X-O-C5),3,4-epoxybutyl beta-D-xyloside (X-O-C4), and 2,3-epoxypropyl beta-D-xyloside (X-O-C3) were determined by X-ray crystallography. High-resolution measurement revealed clear electron densities for each ligand. Both X-O-C5 and X-O-C3 were found to form a covalent bond with the putative nucleophile Glu86. Unexpectedly, X-O-C4 was found to bind to the putative acid/base catalyst Glu177. In all three complexes, clear conformational changes were found in XYNII compared to the native structure. These changes were largest in the X-O-C3 complex structure.
PDB ID: 1REDDownload
MMDB ID: 5307
PDB Deposition Date: 1995/12/21
Updated in MMDB: 1998/11
Experimental Method:
x-ray diffraction
Resolution: 1.6  Å
Source Organism:
Similar Structures:
Biological Unit for 1RED: monomeric; determined by author
Molecular Components in 1RED
Label Count Molecule
Protein (1 molecule)
Endo-1,4-beta-xylanase II
Molecule annotation
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Citing MMDB