1QK7: Solution structure of Selenocosmia huwena lectin-I(SHL-I) by 2D-NMR

The three-dimensional structure of native SHL-I, a lectin from the venom of the Chinese bird spider Selenocosmia huwena, has been determined from two-dimensional 1H NMR spectroscopy recorded at 500 and 600 MHz. The best 10 structures have NOE violation <0.3 A, dihedral violation <2 deg, and average root-mean-square differences of 0.85 + 0.06 A over backbone atoms. The structure consists of a three-stranded antiparallel beta-sheet and three turns. The three disulfide bridges and three-stranded antiparallel beta-sheet form a inhibitor cystine knot motif which is adopted by several other small proteins, such as huwentoxin-I, omega-conotoxin, and gurmarin. The C-terminal fragment from Leu28 to Trp32 adopts two sets of conformations corresponding to the cis and trans conformations of Pro31. The structure of SHL-I also has high similarity with that of the N-terminus of hevein, a lectin from rubber-tree latex.
PDB ID: 1QK7Download
MMDB ID: 13306
PDB Deposition Date: 1999/7/10
Updated in MMDB: 2007/11
Experimental Method:
solution nmr
Source Organism:
Similar Structures:
Biological Unit for 1QK7: monomeric; determined by author
Molecular Components in 1QK7
Label Count Molecule
Protein (1 molecule)
Selenocosmia Huwena Lectin-i
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB