1QCS: N-Terminal Domain Of N-Ethylmaleimide Sensitive Factor (Nsf)

Citation:
Abstract
N-ethylmaleimide-sensitive factor (NSF) is a hexameric ATPase essential for eukaryotic vesicle fusion. Along with SNAP proteins, it disassembles cis-SNARE complexes upon ATP hydrolysis, preparing SNAREs for trans complex formation. We have determined the crystal structure of the N-terminal domain of NSF (N) to 1.9 A resolution. N contains two subdomains which form a groove that is a likely SNAP interaction site. Unexpectedly, both N subdomains are structurally similar to domains in EF-Tu. Based on this similarity, we propose a model for a large conformational change in NSF that drives SNARE complex disassembly.
PDB ID: 1QCSDownload
MMDB ID: 12310
PDB Deposition Date: 1999/5/14
Updated in MMDB: 2007/10
Experimental Method:
x-ray diffraction
Resolution: 1.9  Å
Source Organism:
Similar Structures:
Biological Unit for 1QCS: monomeric; determined by author
Molecular Components in 1QCS
Label Count Molecule
Protein (1 molecule)
1
N-ethylmaleimide Sensitive Factor (Nsf-n)
Molecule annotation
Chemicals (3 molecules)
1
3
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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