1Q9M: Three Dimensional Structures Of Pde4d In Complex With Roliprams And Implication On Inhibitor Selectivity

Citation:
Abstract
Selective inhibitors against the 11 families of cyclic nucleotide phosphodiesterases (PDEs) are used to treat various human diseases. How the inhibitors selectively bind the conserved PDE catalytic domains is unknown. The crystal structures of the PDE4D2 catalytic domain in complex with (R)- or (R,S)-rolipram suggest that inhibitor selectivity is determined by the chemical nature of amino acids and subtle conformational changes of the binding pockets. The conformational states of Gln369 in PDE4D2 may play a key role in inhibitor recognition. The corresponding Y329S mutation in PDE7 may lead to loss of the hydrogen bonds between rolipram and Gln369 and is thus a possible reason explaining PDE7's insensitivity to rolipram inhibition. Docking of the PDE5 inhibitor sildenafil into the PDE4 catalytic pocket further helps understand inhibitor selectivity.
PDB ID: 1Q9MDownload
MMDB ID: 23941
PDB Deposition Date: 2003/8/25
Updated in MMDB: 2007/10
Experimental Method:
x-ray diffraction
Resolution: 2.3  Å
Source Organism:
Similar Structures:
Biological Unit for 1Q9M: tetrameric; determined by author
Molecular Components in 1Q9M
Label Count Molecule
Proteins (4 molecules)
4
Camp-specific Phosphodiesterase Pde4d2(Gene symbol: PDE4D)
Molecule annotation
Chemicals (12 molecules)
1
8
2
4
* Click molecule labels to explore molecular sequence information.

Citing MMDB
.