1Q66: Crystal Structure Of Tgt In Complex With 2-Amino-6-Aminomethyl-8- Phenylsulfanylmethyl-3h-Quinazolin-4-One Crystallized At Ph 5.5

Citation:
Abstract
The enzyme tRNA-guanine transglycosylase (TGT) is involved in the pathogenicity of Shigellae. As the crystal structure of this protein is known, it is a putative target for the structure-based design of inhibitors. Here we report a crystallographic study of several new ligands exhibiting a 2,6-diamino-3H-quinazolin-4-one scaffold, which has been shown recently to be a promising template for TGT-inhibitors. Crystal structure analysis of these complexes has revealed an unexpected movement of the side-chain of Asp102. A detailed analysis of the water network disrupted by this rotation has lead to the derivation of a new composite pharmacophore. A virtual screening has been performed based on this pharmacophore hypothesis and several new inhibitors of micromolar binding affinity with new skeletons have been discovered.
PDB ID: 1Q66Download
MMDB ID: 27286
PDB Deposition Date: 2003/8/12
Updated in MMDB: 2012/12
Experimental Method:
x-ray diffraction
Resolution: 1.75  Å
Source Organism:
Similar Structures:
Biological Unit for 1Q66: monomeric; determined by author
Molecular Components in 1Q66
Label Count Molecule
Protein (1 molecule)
1
Queuine tRNA-ribosyltransferase
Molecule annotation
Chemicals (2 molecules)
1
1
2
1
* Click molecule labels to explore molecular sequence information.

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