1Q09: Crystal Structure Of The Zn(Ii) Form Of E. Coli Zntr, A Zinc-Sensing Transcriptional Regulator (Space Group I4122)

Citation:
Abstract
The earliest of a series of copper efflux genes in Escherichia coli are controlled by CueR, a member of the MerR family of transcriptional activators. Thermodynamic calibration of CueR reveals a zeptomolar (10(-21) molar) sensitivity to free Cu+, which is far less than one atom per cell. Atomic details of this extraordinary sensitivity and selectivity for +1transition-metal ions are revealed by comparing the crystal structures of CueR and a Zn2+-sensing homolog, ZntR. An unusual buried metal-receptor site in CueR restricts the metal to a linear, two-coordinate geometry and uses helix-dipole and hydrogen-bonding interactions to enhance metal binding. This binding mode is rare among metalloproteins but well suited for an ultrasensitive genetic switch.
PDB ID: 1Q09Download
MMDB ID: 24812
PDB Deposition Date: 2003/7/15
Updated in MMDB: 2012/12
Experimental Method:
x-ray diffraction
Resolution: 2.5  Å
Source Organism:
Similar Structures:
Biological Unit for 1Q09: dimeric; determined by author and by software (PISA,PQS)
Molecular Components in 1Q09
Label Count Molecule
Proteins (2 molecules)
2
Zn(ii)-responsive Regulator of Znta(Gene symbol: zntR)
Molecule annotation
Chemicals (10 molecules)
1
6
2
4
* Click molecule labels to explore molecular sequence information.

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