1P8X: The Calcium-activated C-terminal Half Of Gelsolin

Gelsolin requires activation to carry out its severing and capping activities on F-actin. Here, we present the structure of the isolated C-terminal half of gelsolin (G4-G6) at 2.0 A resolution in the presence of Ca(2+) ions. This structure completes a triptych of the states of activation of G4-G6 that illuminates its role in the function of gelsolin. Activated G4-G6 displays an open conformation, with the actin-binding site on G4 fully exposed and all three type-2 Ca(2+) sites occupied. Neither actin nor the type-l Ca(2+), which normally is sandwiched between actin and G4, is required to achieve this conformation.
PDB ID: 1P8XDownload
MMDB ID: 25050
PDB Deposition Date: 2003/5/8
Updated in MMDB: 2003/11
Experimental Method:
x-ray diffraction
Resolution: 2  Å
Source Organism:
Similar Structures:
Biological Unit for 1P8X: monomeric; determined by author
Molecular Components in 1P8X
Label Count Molecule
Protein (1 molecule)
Gelsolin Precursor, Plasma(Gene symbol: GSN)
Molecule annotation
Chemicals (3 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB