1OGF: The Structure Of Bacillus Subtilis Rbsd Complexed With Glycerol

Citation:
Abstract
RbsD is the only protein whose biochemical function is unknown among the six gene products of the rbs operon involved in the active transport of ribose. FucU, a paralogue of RbsD conserved from bacteria to human, is also the only protein whose function is unknown among the seven gene products of the l-fucose regulon. Here we report the crystal structures of Bacillus subtilis RbsD, which reveals a novel decameric toroidal assembly of the protein. Nuclear magnetic resonance and other studies on RbsD reveal that the intersubunit cleft of the protein binds specific forms of d-ribose, but it does not have an enzyme activity toward the sugar. Likewise, FucU binds l-fucose but lacks an enzyme activity toward this sugar. We conclude that RbsD and FucU are cytoplasmic sugar-binding proteins, a novel class of proteins whose functional role may lie in helping influx of the sugar substrates.
PDB ID: 1OGFDownload
MMDB ID: 23951
PDB Deposition Date: 2003/4/30
Updated in MMDB: 2007/10
Experimental Method:
x-ray diffraction
Resolution: 2.3  Å
Source Organism:
Similar Structures:
Biological Unit for 1OGF: decameric; determined by author and by software (PQS)
Molecular Components in 1OGF
Label Count Molecule
Proteins (10 molecules)
10
High Affinity Ribose Transport Protein Rbsd(Gene symbol: rbsD)
Molecule annotation
Chemicals (14 molecules)
1
10
2
4
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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