1OCB: Structure Of The Wild-Type Cellobiohydrolase Cel6a From Humicolas Insolens In Complex With A Fluorescent Substrate

Citation:
Abstract
The enzymatic digestion of cellulose entails intimate involvement of cellobiohydrolases, whose characteristic active-center tunnel contributes to a processive degradation of the polysaccharide. The cellobiohydrolase Cel6A displays an active site within a tunnel formed by two extended loops, which are known to open and close in response to ligand binding. Here we present five structures of wild-type and mutant forms of Cel6A from Humicola insolens in complex with nonhydrolyzable thio-oligosaccharides, at resolutions from 1.7-1.1 A, dissecting the structural accommodation of a processing substrate chain through the active center during hydrolysis. Movement of ligand is facilitated by extensive solvent-mediated interactions and through flexibility in the hydrophobic surfaces provided by a sheath of tryptophan residues.
PDB ID: 1OCBDownload
MMDB ID: 24508
PDB Deposition Date: 2003/2/7
Updated in MMDB: 2012/11
Experimental Method:
x-ray diffraction
Resolution: 1.75  Å
Source Organism:
Similar Structures:
Biological Unit for 1OCB: monomeric; determined by author and by software (PQS)
Molecular Components in 1OCB
Label Count Molecule
Protein (1 molecule)
1
Cellobiohydrolase II
Molecule annotation
Chemicals (9 molecules)
1
1
2
2
3
4
4
1
5
1
* Click molecule labels to explore molecular sequence information.

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