1O1O: Deoxy Hemoglobin (A,C:v1m,V62l; B,D:v1m,V67l)

Acta Crystallogr. D Biol. Crystallogr. (2000) 56 p.812-816» All references (3)
The crystal structures of three recombinant human hemoglobins, rHb1. 0, rHb1.1 and rHb1.2, have been determined in the deoxy state at 1.8 A resolution. Two of the three proteins, rHb1.1 and rHb1.2, contain a genetic fusion of the alpha subunits, a one- or two-glycine link, respectively, whereas rHb1.0 does not. The glycine crosslinks, localized between one N- and C--termini pair of the alpha subunits in the deoxy crystalline state, do not perturb the overall tertiary or quaternary or even the local structure of hemoglobin. Therefore, genetic fusion to prevent the dissociation of the hemoglobin tetramer, thereby inhibiting renal clearance based upon molecular size, is a structurally conservative method to stabilize hemoglobin for use as an oxygen-delivery therapeutic.
PDB ID: 1O1ODownload
MMDB ID: 21560
PDB Deposition Date: 2002/11/17
Updated in MMDB: 2007/10
Experimental Method:
x-ray diffraction
Resolution: 1.8  Å
Source Organism:
Similar Structures:
Biological Unit for 1O1O: tetrameric; determined by author and by software (PISA)
Molecular Components in 1O1O
Label Count Molecule
Proteins (4 molecules)
Hemoglobin Alpha Chain(Gene symbol: HBA1)
Molecule annotation
Hemoglobin Beta Chain(Gene symbol: HBB)
Molecule annotation
Chemicals (4 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB