1N7A: Rip-radiation-damage Induced Phasing

The use of third generation synchrotron sources has led to renewed concern about the effect of ionizing radiation on crystalline biological samples. In general, the problem is seen as one to be avoided. However, in this paper, it is shown that, far from being a hindrance to successful structure determination, radiation damage provides an opportunity for phasing macromolecular structures. This is successfully demonstrated for both a protein and an oligonucleotide, by way of which complete models were built automatically. The possibility that, through the exploitation of radiation damage, the phase problem could become less of a barrier to macromolecular crystal structure determination is discussed.
PDB ID: 1N7ADownload
MMDB ID: 50427
PDB Deposition Date: 2002/11/13
Updated in MMDB: 2017/11
Experimental Method:
x-ray diffraction
Resolution: 1.2  Å
Biological Unit for 1N7A: octameric; determined by author
Molecular Components in 1N7A
Label Count Molecule
Nucleotide(1 molecule)
Rna/dna (5'-r(*u)-d(p*(bgm))-r(p*ap*gp*gp*u)-3')
Molecule annotation
Chemicals (11 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB