1N59: Crystal structure of the Murine class I Major Histocompatibility Complex of H-2KB, B2-Microglobulin, and A 9-Residue immunodominant peptide epitope gp33 derived from LCMV

LCMV infection of H-2(b) mice generates a CD8(+) CTL response mainly directed toward three immunodominant epitopes. One of these, gp33, is presented by both H-2D(b) and H-2K(b) MHC class I molecules. The virus can escape immune recognition in the context of both these MHC class I molecules through single mutations of the peptide. In order to understand the underlying structural mechanism, we determined the crystal structures of both complexes. The structures reveal that the peptide is presented in two diametrically opposed manners by H-2D(b) and H-2K(b), with residues used as anchor positions in one MHC class I molecule interacting with the TCR in the other. Importantly, the peptide's N-terminal residue p1K protrudes from the binding cleft in H-2K(b). We present structural evidence that explains the functional consequences of single mutations found in escape variants.
PDB ID: 1N59Download
MMDB ID: 21830
PDB Deposition Date: 2002/11/5
Updated in MMDB: 2007/10
Experimental Method:
x-ray diffraction
Resolution: 2.95  Å
Source Organism:
Mus musculus
Similar Structures:
Biological Unit for 1N59: trimeric; determined by author and by software (PISA)
Molecular Components in 1N59
Label Count Molecule
Proteins (3 molecules)
H-2 Class I Histocompatibility Antigen, K-B Alpha Chain(Gene symbol: H2-K1)
Molecule annotation
Molecule annotation
Nonameric Peptide, Gp33 Derived From Lymphocytic Choriomeningitis Virus
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB