1N3Q: Pterocarpus Angolensis Lectin Complexed With Turanose

Citation:
Abstract
The crystal structure of the Man/Glc-specific seed lectin from Pterocarpus angolensis was determined in complex with methyl-alpha-d-glucose, sucrose, and turanose. The carbohydrate binding site contains a classic Man/Glc type specificity loop. Its metal binding loop on the other hand is of the long type, different from what is observed in other Man/Glc-specific legume lectins. Glucose binding in the primary binding site is reminiscent of the glucose complexes of concanavalin A and lentil lectin. Sucrose is found to be bound in a conformation similar as seen in the binding site of lentil lectin. A direct hydrogen bond between Ser-137(OG) to Fru(O2) in Pterocarpus angolensis lectin replaces a water-mediated interaction in the equivalent complex of lentil lectin. In the turanose complex, the binding site of the first molecule in the asymmetric unit contains the alphaGlc1-3betaFruf form of furanose while the second molecule contains the alphaGlc1-3betaFrup form in its binding site.
PDB ID: 1N3QDownload
MMDB ID: 21284
PDB Deposition Date: 2002/10/29
Updated in MMDB: 2002/12
Experimental Method:
x-ray diffraction
Resolution: 2.2  Å
Source Organism:
Similar Structures:
Biological Unit for 1N3Q: dimeric; determined by author
Molecular Components in 1N3Q
Label Count Molecule
Proteins (2 molecules)
2
Lectin PAL
Molecule annotation
Chemicals (8 molecules)
1
2
2
1
3
2
4
2
5
1
* Click molecule labels to explore molecular sequence information.

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