1MSF: SOLUTION STRUCTURE OF A SPECIFIC DNA COMPLEX OF THE MYB DNA-BINDING DOMAIN WITH COOPERATIVE RECOGNITION HELICES

Citation:
Abstract
The DNA-binding region of Myb consists of three imperfect tandem repeats (R1, R2, and R3). We have determined the solution structure of a specific DNA complex of the minimum DNA-binding domain (R2R3) by heteronuclear multidimensional NMR. Both R2 and R3 contain three helices, and the third helix in each is found to be a recognition helix. R2 and R3 are closely packed in the major groove, so that the two recognition helices contact each other directly to bind to the specific base sequence, AACNG cooperatively; this is a significant arrangement of recognition helices. The three key base pairs in this sequence are specifically recognized by Asn-183 (R3), Lys-182 (R3), and Lys-128 (R2). In contrast, R1 has no specific interactions with DNA from our NMR study of the DNA complex of the full DNA-binding domain (R1R2R3).
PDB ID: 1MSFDownload
MMDB ID: 50355
PDB Deposition Date: 1995/1/24
Updated in MMDB: 2007/11
Experimental Method:
solution nmr
Source Organism:
Mus musculus
Similar Structures:
Molecular Components in 1MSF
Label Count Molecule
Protein (1 molecule)
1
C-myb DNA-binding Domain(Gene symbol: Myb)
Molecule annotation
Nucleotides(2 molecules)
1
DNA (5'-d(*cp*cp*tp*ap*ap*cp*tp*gp*ap*cp*ap*cp*ap*cp*ap*t)-3')
Molecule annotation
1
DNA (5'-d(*ap*tp*gp*tp*gp*tp*gp*tp*cp*ap*gp*tp*tp*ap*gp*g)-3')
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
.