1ML0: Viral Chemokine Binding Protein M3 From Murine Gammaherpesvirus68 In Complex With The P8a Variant Of Cc-Chemokine Mcp-1

The M3 protein encoded by murine gamma herpesvirus68 (gamma HV68) functions as an immune system saboteur by the engagement of chemoattractant cytokines, thereby altering host antiviral inflammatory responses. Here we report the crystal structures of M3 both alone and in complex with the CC chemokine MCP-1. M3 is a two-domain beta sandwich protein with a unique sequence and topology, forming a tightly packed anti-parallel dimer. The stoichiometry of the MCP-1:M3 complex is 2:2, with two monomeric chemokines embedded at distal ends of the preassociated M3 dimer. Conformational flexibility and electrostatic complementation are both used by M3 to achieve high-affinity and broad-spectrum chemokine engagement. M3 also employs structural mimicry to promiscuously sequester chemokines, engaging conservative structural elements associated with both chemokine homodimerization and binding to G protein-coupled receptors.
PDB ID: 1ML0Download
MMDB ID: 21238
PDB Deposition Date: 2002/8/29
Updated in MMDB: 2002/12
Experimental Method:
x-ray diffraction
Resolution: 2.8  Å
Source Organism:
Murid gammaherpesvirus 4
Similar Structures:
Biological Unit for 1ML0: tetrameric; determined by author and by software (PISA)
Molecular Components in 1ML0
Label Count Molecule
Proteins (4 molecules)
M3 Protein
Molecule annotation
Small Inducible Cytokine(Gene symbol: CCL2)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB