1MHQ: Crystal Structure Of Human Gga2 Vhs Domain

Citation:
Abstract
Golgi-localized, gamma-ear-containing, ARF binding (GGA) proteins regulate intracellular vesicle transport by recognizing sorting signals on the cargo surface in the initial step of the budding process. The VHS (VPS27, Hrs, and STAM) domain of GGA binds with the signal peptides. Here, a crystal structure of the VHS domain of GGA2 is reported at 2.2 A resolution, which permits a direct comparison with that of homologous proteins, GGA1 and GGA3. Significant structural difference is present in the loop between helices 6 and 7, which forms part of the ligand binding pocket. Intrinsic fluorescence spectroscopic study indicates that this loop undergoes a conformational change upon ligand binding. Thus, the current structure suggests that a conformational change induced by ligand binding occurs in this part of the ligand pocket.
PDB ID: 1MHQDownload
MMDB ID: 22332
PDB Deposition Date: 2002/8/20
Updated in MMDB: 2017/11
Experimental Method:
x-ray diffraction
Resolution: 2.2  Å
Source Organism:
Similar Structures:
Biological Unit for 1MHQ: monomeric; determined by author
Molecular Components in 1MHQ
Label Count Molecule
Protein (1 molecule)
1
Adp-ribosylation Factor Binding Protein Gga2(Gene symbol: GGA2)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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