1M4P: Structure of the Tsg101 UEV domain in complex with a HIV-1 PTAP "late domain" peptide, DYANA Ensemble

The structural proteins of HIV and Ebola display PTAP peptide motifs (termed 'late domains') that recruit the human protein Tsg101 to facilitate virus budding. Here we present the solution structure of the UEV (ubiquitin E2 variant) binding domain of Tsg101 in complex with a PTAP peptide that spans the late domain of HIV-1 p6(Gag). The UEV domain of Tsg101 resembles E2 ubiquitin-conjugating enzymes, and the PTAP peptide binds in a bifurcated groove above the vestigial enzyme active site. Each PTAP residue makes important contacts, and the Ala 9-Pro 10 dipeptide binds in a deep pocket of the UEV domain that resembles the X-Pro binding pockets of SH3 and WW domains. The structure reveals the molecular basis of HIV PTAP late domain function and represents an attractive starting point for the design of novel inhibitors of virus budding.
PDB ID: 1M4PDownload
MMDB ID: 20953
PDB Deposition Date: 2002/7/3
Updated in MMDB: 2007/11
Experimental Method:
solution nmr
Source Organism:
Human immunodeficiency virus 1
Similar Structures:
Molecular Components in 1M4P
Label Count Molecule
Proteins (2 molecules)
Tumor Susceptibility Gene 101 Protein(Gene symbol: TSG101)
Molecule annotation
GAG Polyprotein
Molecule annotation
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Citing MMDB