1M2R: Crystal Structure of 5,8-di-amino-1,4-di-hydroxy- Anthraquinoneck2 Kinase Complex

Protein kinases play key roles in signal transduction and therefore are among the most attractive targets for drug design. The pharmacological aptitude of protein kinase inhibitors is highlighted by the observation that various diseases with special reference to cancer are because of the abnormal expression/activity of individual kinases. The resolution of the three-dimensional structure of the target kinase in complex with inhibitors is often the starting point for the rational design of this kind of drugs, some of which are already in advanced clinical trial or even in clinical practice. Here we present and discuss three new crystal structures of ATP site-directed inhibitors in complex with "casein kinase-2" (CK2), a constitutively active protein kinase implicated in a variety of cellular functions and misfunctions. With the help of theoretical calculations, we disclose some key features underlying the inhibitory efficiency of anthraquinone derivatives, outlining three different binding modes into the active site. In particular, we show that a nitro group in a hydroxyanthraquinone scaffold decreases the inhibitory constants K(i) because of electron-withdrawing and resonance effects that enhance the polarization of hydroxylic substituents in paraposition.
PDB ID: 1M2RDownload
MMDB ID: 23384
PDB Deposition Date: 2002/6/25
Updated in MMDB: 2012/11
Experimental Method:
x-ray diffraction
Resolution: 1.7  Å
Source Organism:
Similar Structures:
Biological Unit for 1M2R: monomeric; determined by author
Molecular Components in 1M2R
Label Count Molecule
Protein (1 molecule)
Casein Kinase II, Alpha Chain(Gene symbol: gpm388c)
Molecule annotation
Chemical (1 molecule)
* Click molecule labels to explore molecular sequence information.

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