1LK2: 1.35a Crystal Structure of H-2kb Complexed With the Gnysfyal Peptide

Citation:
Abstract
We identify and consider some characteristics of a peptide antagonist for the Ag-specific receptor on 2C cells (the 2C TCR). The peptide, GNYSFYAL (called GNY), binds to H-2K(b), and a very high-resolution crystal structure of the GNY-K(b) complex at 1.35 A is described. Although the GNY peptide does not bind to L(d), the potency of GNY-K(b) as an antagonist is evident from its ability to specifically inhibit 2C TCR-mediated reactions to an allogenic agonist complex (QLSPFPFDL-L(d)), as well as to a syngeneic agonist complex (SIYRYYGL-K(b)). The crystal structure and the activities of alanine-substituted peptide variants point to the properties of the peptide P4 side chain and the conformation of the Tyr-P6 side chain as the structural determinants of GNYSFYAL antagonist activity.
PDB ID: 1LK2Download
MMDB ID: 25229
PDB Deposition Date: 2002/4/23
Updated in MMDB: 2003/12 
Experimental Method:
x-ray diffraction
Resolution: 1.35  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 1LK2: trimeric; determined by author and by software (PISA)
Molecular Components in 1LK2
Label Count Molecule
Proteins (3 molecules)
1
H-2 Class I Histocompatibility Antigen, K-B Alpha Chain
Molecule annotation
1
Beta-2-microglobulin
Molecule annotation
1
Insulin Receptor, Beta-subunit
Molecule annotation
Chemicals (7 molecules)
1
2
2
1
3
1
4
2
5
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
.