1LJZ: NMR structure of an AChR-peptide (Torpedo Californica, alpha-subunit residues 182-202) in complex with alpha-Bungarotoxin

The structure of a peptide corresponding to residues 182-202 of the acetylcholine receptor alpha1 subunit in complex with alpha-bungarotoxin was solved using NMR spectroscopy. The peptide contains the complete sequence of the major determinant of AChR involved in alpha-bungarotoxin binding. One face of the long beta hairpin formed by the AChR peptide consists of exposed nonconserved residues, which interact extensively with the toxin. Mutations of these receptor residues confer resistance to the toxin. Conserved AChR residues form the opposite face of the beta hairpin, which creates the inner and partially hidden pocket for acetylcholine. An NMR-derived model for the receptor complex with two alpha-bungarotoxin molecules shows that this pocket is occupied by the conserved alpha-neurotoxin residue R36, which forms cation-pi interactions with both alphaW149 and gammaW55/deltaW57 of the receptor and mimics acetylcholine.
PDB ID: 1LJZDownload
MMDB ID: 20238
PDB Deposition Date: 2002/4/23
Updated in MMDB: 2007/11
Experimental Method:
solution nmr
Source Organism:
Bungarus multicinctus
Similar Structures:
Biological Unit for 1LJZ: dimeric; determined by author
Molecular Components in 1LJZ
Label Count Molecule
Proteins (2 molecules)
Molecule annotation
Acetylcholine Receptor Protein
Molecule annotation
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Citing MMDB