1KQ4: Crystal Structure Of A Thy1-complementing Protein (tm0449) From Thermotoga Maritima At 2.25 A Resolution

Structural genomics is emerging as a principal approach to define protein structure-function relationships. To apply this approach on a genomic scale, novel methods and technologies must be developed to determine large numbers of structures. We describe the design and implementation of a high-throughput structural genomics pipeline and its application to the proteome of the thermophilic bacterium Thermotoga maritima. By using this pipeline, we successfully cloned and attempted expression of 1,376 of the predicted 1,877 genes (73%) and have identified crystallization conditions for 432 proteins, comprising 23% of the T. maritima proteome. Representative structures from TM0423 glycerol dehydrogenase and TM0449 thymidylate synthase-complementing protein are presented as examples of final outputs from the pipeline.
PDB ID: 1KQ4Download
MMDB ID: 71242
PDB Deposition Date: 2002/1/3
Updated in MMDB: 2017/11
Experimental Method:
x-ray diffraction
Resolution: 2.25  Å
Source Organism:
Similar Structures:
Biological Unit for 1KQ4: tetrameric; determined by author and by software (PISA)
Molecular Components in 1KQ4
Label Count Molecule
Proteins (4 molecules)
Hypothetical Protein Tm0449(Gene symbol: thyX)
Molecule annotation
Chemicals (4 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB