1JW1: Crystallization And Structure Determination Of Goat Lactoferrin At 4.0 Resolution: A New Form Of Packing In Lactoferrins With A High Solvent Content In Crystals

Citation:
Abstract
Lactoferrin was purified from fresh samples of goat colostrums, saturated with Fe3+ and CO3(2-) ions and crystallized by microdialysis method. The crystals belong to orthorhombic space group P2(1)2(1)2(1) with a=104.6 A, b=153.8 A, c=155.1 A and Z=4. The quality of crystals was poor, thus the intensity data were restricted to 4.0 A resolution only. The structure was determined by molecular replacement method using diferric buffalo lactoferrin as a model. The solution clearly indicated the presence of one molecule in the asymmetric unit, which corresponds to a Vm value of 7.1 A3/Da. The structure was refined with stringent constraints to an R-factor of 0.246 using all the reflections 15,870 to 4.0 A resolution. The overall structure of goat lactoferrin is essentially similar to those of buffalo and bovine lactoferrins. However, the iron-binding environment in goat lactoferrin is somewhat different, in which 2 CO3(2-). ions have low occupancies. The solvent content of approximately 84% was very high in the present case which explains the fragility of the crystals of goat lactoferrin. In a way, it is very surprising that the crystals grow at all, although crystals with solvent as high as 89% have been reported.
PDB ID: 1JW1Download
MMDB ID: 22582
PDB Deposition Date: 2001/9/2
Updated in MMDB: 2012/12
Experimental Method:
x-ray diffraction
Resolution: 4  Å
Source Organism:
Similar Structures:
Biological Unit for 1JW1: monomeric; determined by author
Molecular Components in 1JW1
Label Count Molecule
Protein (1 molecule)
1
Lactoferrin(Gene symbol: LTF)
Molecule annotation
Chemicals (2 molecules)
1
2
* Click molecule labels to explore molecular sequence information.

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