1JUP: Crystal Structure Of The Multidrug Binding Transcriptional Repressor Qacr Bound To Malachite Green

The Staphylococcus aureus multidrug binding protein QacR represses transcription of the qacA multidrug transporter gene and is induced by structurally diverse cationic lipophilic drugs. Here, we report the crystal structures of six QacR-drug complexes. Compared to the DNA bound structure, drug binding elicits a coil-to-helix transition that causes induction and creates an expansive multidrug-binding pocket, containing four glutamates and multiple aromatic and polar residues. These structures indicate the presence of separate but linked drug-binding sites within a single protein. This multisite drug-binding mechanism is consonant with studies on multidrug resistance transporters.
PDB ID: 1JUPDownload
MMDB ID: 18165
PDB Deposition Date: 2001/8/24
Updated in MMDB: 2001/12
Experimental Method:
x-ray diffraction
Resolution: 2.95  Å
Source Organism:
Similar Structures:
Biological Unit for 1JUP: dimeric; determined by author and by software (PISA)
Molecular Components in 1JUP
Label Count Molecule
Proteins (2 molecules)
Hypothetical Transcriptional Regulator in Qaca 5'region(Gene symbol: qacR)
Molecule annotation
Chemicals (12 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB