1JMZ: Crystal Structure Of A Quinohemoprotein Amine Dehydrogenase From Pseudomonas Putida With Inhibitor

Citation:
Abstract
The crystal structure of a quinohemoprotein amine dehydrogenase from Pseudomonas putida has been determined at 1.9-A resolution. The enzyme comprises three non-identical subunits: a four-domain alpha-subunit that harbors a di-heme cytochrome c, a seven-bladed beta-propeller beta-subunit that provides part of the active site, and a small gamma-subunit that contains a novel cross-linked, proteinous quinone cofactor, cysteine tryptophylquinone. More surprisingly, the catalytic gamma-subunit contains three additional chemical cross-links that encage the cysteine tryptophylquinone cofactor, involving a cysteine side chain bridged to either an Asp or Glu residue all in a hitherto unknown thioether bonding with a methylene carbon atom of acidic amino acid side chains. Thus, the structure of the 79-residue gamma-subunit is quite unusual, containing four internal cross-links in such a short polypeptide chain that would otherwise be difficult to fold into a globular structure.
PDB ID: 1JMZDownload
MMDB ID: 18571
PDB Deposition Date: 2001/7/20
Updated in MMDB: 2007/10
Experimental Method:
x-ray diffraction
Resolution: 2  Å
Source Organism:
Similar Structures:
Biological Unit for 1JMZ: trimeric; determined by author and by software (PISA)
Molecular Components in 1JMZ
Label Count Molecule
Proteins (3 molecules)
1
Amine Dehydrogenase
Molecule annotation
1
Amine Dehydrogenase
Molecule annotation
1
Amine Dehydrogenase
Molecule annotation
Chemicals (4 molecules)
1
1
2
2
3
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
.