1JMT: X-Ray Structure Of A Core U2af65U2AF35 HETERODIMER

U2 auxiliary factor (U2AF) is an essential splicing factor that recognizes the 3' splice site and recruits the U2 snRNP to the branch point. The X-ray structure of the human core U2AF heterodimer, consisting of the U2AF35 central domain and a proline-rich region of U2AF65, has been determined at 2.2 A resolution. The structure reveals a novel protein-protein recognition strategy, in which an atypical RNA recognition motif (RRM) of U2AF35 and the U2AF65 polyproline segment interact via reciprocal "tongue-in-groove" tryptophan residues. Complementary biochemical experiments demonstrate that the core U2AF heterodimer binds RNA, and that the interacting tryptophan side chains are essential for U2AF dimerization. Atypical RRMs in other splicing factors may serve as protein-protein interaction motifs elsewhere during spliceosome assembly.
PDB ID: 1JMTDownload
MMDB ID: 17361
PDB Deposition Date: 2001/7/19
Updated in MMDB: 2007/10
Experimental Method:
x-ray diffraction
Resolution: 2.2  Å
Source Organism:
Similar Structures:
Biological Unit for 1JMT: dimeric; determined by author and by software (PISA)
Molecular Components in 1JMT
Label Count Molecule
Proteins (2 molecules)
Splicing Factor U2af 35 KDA Subunit(Gene symbol: U2AF1)
Molecule annotation
Splicing Factor U2af 65 KDA Subunit(Gene symbol: U2AF2)
Molecule annotation
Chemicals (2 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB