1JGX: Photosynthetic Reaction Center Mutant With Thr M 21 Replaced With Asp

Citation:
Abstract
The role of contact interactions in the crystallization of membrane proteins was assessed by mutation of amino-acid residues on the surface of the reaction center from Rhodobacter sphaeroides. Five single-site mutants were constructed, with changes in contact regions found in the trigonal and tetragonal forms but not the orthorhombic form. Crystallization trials for the tetragonal form yielded either no crystals or crystals with an altered morphology, whereas crystals grew in the other two forms, indicating that these interactions are essential for the stability of the tetragonal crystals. Changes in the structures determined by X-ray diffraction of trigonal crystals for each mutant were related to the quality of the diffraction. Significant differences in the resolution limit of the crystals were associated with the loss of specific interactions between neighboring proteins. The results suggest that the contact regions are crucial for obtaining highly ordered crystals of membrane proteins.
PDB ID: 1JGXDownload
MMDB ID: 17064
PDB Deposition Date: 2001/6/27
Updated in MMDB: 2007/10
Experimental Method:
x-ray diffraction
Resolution: 3.01  Å
Source Organism:
Similar Structures:
Biological Unit for 1JGX: trimeric; determined by author and by software (PISA)
Molecular Components in 1JGX
Label Count Molecule
Proteins (3 molecules)
1
Photosynthetic Reaction Center L Subunit
Molecule annotation
1
Photosynthetic Reaction Center M Subunit
Molecule annotation
1
Photosynthetic Reaction Center H Subunit
Molecule annotation
Chemicals (9 molecules)
1
4
2
2
3
1
4
1
5
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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