1JGN: Solution structure of the C-terminal PABC domain of human poly(A)-binding protein in complex with the peptide from Paip2

Citation:
Abstract
The C-terminal domain of poly(A)-binding protein (PABC) is a peptide-binding domain found in poly(A)-binding proteins (PABPs) and a HECT (homologous to E6-AP C-terminus) family E3 ubiquitin ligase. In protein synthesis, the PABC domain of PABP functions to recruit several translation factors possessing the PABP-interacting motif 2 (PAM2) to the mRNA poly(A) tail. We have determined the solution structure of the human PABC domain in complex with two peptides from PABP-interacting protein-1 (Paip1) and Paip2. The structures show a novel mode of peptide recognition, in which the peptide binds as a pair of beta-turns with extensive hydrophobic, electrostatic and aromatic stacking interactions. Mutagenesis of PABC and peptide residues was used to identify key protein-peptide interactions and quantified by isothermal calorimetry, surface plasmon resonance and GST pull-down assays. The results provide insight into the specificity of PABC in mediating PABP-protein interactions.
PDB ID: 1JGNDownload
MMDB ID: 23317
PDB Deposition Date: 2001/6/26
Updated in MMDB: 2007/11
Experimental Method:
solution nmr
Source Organism:
Similar Structures:
Molecular Components in 1JGN
Label Count Molecule
Proteins (2 molecules)
1
Polyadenylate-binding Protein 1(Gene symbol: PABPC1)
Molecule annotation
1
Polyadenylate-binding Protein-interacting Protein 2(Gene symbol: PAIP2)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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