1J4W: COMPLEX OF THE KH3 and KH4 DOMAINS OF FBP WITH A SINGLE_STRANDED 29mer DNA OLIGONUCLEOTIDE FROM THE FUSE ELEMENT OF THE C-MYC ONCOGENE

Citation:
Abstract
Gene regulation can be tightly controlled by recognition of DNA deformations that are induced by stress generated during transcription. The KH domains of the FUSE-binding protein (FBP), a regulator of c-myc expression, bind in vivo and in vitro to the single-stranded far-upstream element (FUSE), 1,500 base pairs upstream from the c-myc promoter. FBP bound to FUSE acts through TFIIH at the promoter. Here we report the solution structure of a complex between the KH3 and KH4 domains of FBP and a 29-base single-stranded DNA from FUSE. The KH domains recognize two sites, 9-10 bases in length, separated by 5 bases, with KH4 bound to the 5' site and KH3 to the 3' site. The central portion of each site comprises a tetrad of sequence 5'd-ATTC for KH4 and 5'd-TTTT for KH3. Dynamics measurements show that the two KH domains bind as articulated modules to single-stranded DNA, providing a flexible framework with which to recognize transient, moving targets.
PDB ID: 1J4WDownload
MMDB ID: 49727
PDB Deposition Date: 2001/11/30
Updated in MMDB: 2007/11
Experimental Method:
solution nmr
Source Organism:
Homo sapiens
Similar Structures:
Molecular Components in 1J4W
Label Count Molecule
Protein (1 molecule)
1
Fuse Binding Protein(Gene symbol: FUBP1)
Molecule annotation
Nucleotide(1 molecule)
1
DNA (5'-d(*gp*tp*a*tp*ap*tp*tp*cp*cp*cp*tp*cp*gp*gp*g*ap*tp*tp*tp*tp*tp*tp*ap*tp*tp*tp*tp*gp*t)-3')
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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