1IG3: Mouse Thiamin Pyrophosphokinase Complexed With Thiamin

Citation:
Abstract
Thiamin pyrophosphate (TPP) is a coenzyme derived from vitamin B1 (thiamin). TPP synthesis in eukaryotes requires thiamin pyrophosphokinase (TPK), which catalyzes the transfer of a pyrophosphate group from ATP to thiamin. TPP is essential for central metabolic processes, including the formation of acetyl CoA from glucose and the Krebs cycle. Deficiencies in human thiamin metabolism result in beriberi and Wernicke encephalopathy. The crystal structure of mouse TPK was determined by multiwavelength anomalous diffraction at 2.4 A resolution, and the structure of TPK complexed with thiamin has been refined at 1.9 A resolution. The TPK polypeptide folds as an alpha/beta-domain and a beta-sandwich domain, which share a central ten-stranded mixed beta-sheet. TPK subunits associate as a dimer, and thiamin is bound in the dimer interface. Despite lacking apparent sequence homology with other proteins, the alpha/beta-domain resembles the Rossman fold and is similar to other kinase structures, including another pyrophosphokinase and a thiamin biosynthetic enzyme. Comparison of mouse and yeast TPK structures reveals differences that could be exploited in developing species-specific inhibitors of potential use as antimicrobial agents.
PDB ID: 1IG3Download
MMDB ID: 16223
PDB Deposition Date: 2001/4/16
Updated in MMDB: 2007/10
Experimental Method:
x-ray diffraction
Resolution: 1.9  Å
Source Organism:
Similar Structures:
Biological Unit for 1IG3: dimeric; determined by author and by software (PISA)
Molecular Components in 1IG3
Label Count Molecule
Proteins (2 molecules)
2
Thiamin Pyrophosphokinase(Gene symbol: Tpk1)
Molecule annotation
Chemicals (16 molecules)
1
14
2
2
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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