1I6L: 1.7 High Resolution Experimental Phases For Tryptophanyl-Trna Synthetase Complexed With Tryptophanyl-5'amp

Citation:
Abstract
Native data, anomalous data at three wavelengths and an independent peak-wavelength data set for SeMet-substituted protein have been collected from cryoprotected crystals of the TrpRS-adenylate product (TAM) complex to a resolution limit of 1.7 A. Independent phase sets were developed using SHARP and improved by solvent flipping with SOLOMON using molecular envelopes derived from experimental densities for, respectively, peak-wavelength SAD data from four different crystals, MAD data and their M(S)IRAS combinations with native data. Hendrickson-Lattman phase-probability coefficients from each phase set were used in BUSTER to drive maximum-likelihood refinements of well defined parts of the previously refined room-temperature 2.9 A structure. Maximum-entropy completion followed by manual rebuilding was then used to generate a model for the missing segments, bound ligand and solvent molecules. Surprisingly, peak-wavelength SAD experiments produced the smallest phase errors relative to the refined structures. Selenomethionylated models deviate from one another by 0.25 A and from the native model by 0.38 A, but all have r.m.s. deviations of approximately 1.0 A from the 2.9 A model. Difference Fourier calculations between amplitudes from the 300 K experiment and the new amplitudes at 100 K using 1.7 A model phases show no significant structural changes arising from temperature variation or addition of cryoprotectant. The main differences between low- and high-resolution structures arise from correcting side-chain rotamers in the core of the protein as well as on the surface. These changes improve various structure-validation criteria.
PDB ID: 1I6LDownload
MMDB ID: 17559
PDB Deposition Date: 2001/3/2
Updated in MMDB: 2007/11
Experimental Method:
x-ray diffraction
Resolution: 1.72  Å
Source Organism:
Similar Structures:
Biological Unit for 1I6L: dimeric; determined by author and by software (PISA,PQS)
Molecular Components in 1I6L
Label Count Molecule
Proteins (2 molecules)
2
Tryptophanyl-trna Synthetase
Molecule annotation
Chemicals (30 molecules)
1
14
2
2
3
2
4
12
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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