1HW2: Fadr-Dna Complex: Transcriptional Control Of Fatty Acid Metabolism In Echerichia Coli

In Escherichia coli, the expression of fatty acid metabolic genes is controlled by the transcription factor, FadR. The affinity of FadR for DNA is controlled by long chain acyl-CoA molecules, which bind to the protein and modulate gene expression. The crystal structure of FadR reveals a two domain dimeric molecule where the N-terminal domains bind DNA, and the C-terminal domains bind acyl-CoA. The DNA binding domain has a winged-helix motif, and the C-terminal domain resembles the sensor domain of the Tet repressor. The FadR.DNA complex reveals how the protein interacts with DNA and specifically recognizes a palindromic sequence. Structural and functional similarities to the Tet repressor and the BmrR transcription factors suggest how the binding of the acyl-CoA effector molecule to the C-terminal domain may affect the DNA binding affinity of the N-terminal domain. We suggest that the binding of acyl-CoA disrupts a buried network of charged and polar residues in the C-terminal domain, and the resulting conformational change is transmitted to the N-terminal domain via a domain-spanning alpha-helix.
PDB ID: 1HW2Download
MMDB ID: 72321
PDB Deposition Date: 2001/1/9
Updated in MMDB: 2011/06
Experimental Method:
x-ray diffraction
Resolution: 3.25  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 1HW2: tetrameric; determined by author
Molecular Components in 1HW2
Label Count Molecule
Proteins (2 molecules)
Fatty Acid Metabolism Regulator Protein(Gene symbol: fadR)
Molecule annotation
Nucleotides(2 molecules)
5'- D(*cp*gp*ap*tp*cp*tp*gp*gp*tp*cp*cp*gp*ap*cp*cp*ap*gp*ap*tp *gp*cp*t)-3'
Molecule annotation
5'- D(*g*cp*ap*tp*cp*tp*gp*gp*tp*cp*gp*gp*ap*cp*cp*ap*gp*ap*tp* Cp*gp*a)-3'
Molecule annotation
Chemical (1 molecule)
* Click molecule labels to explore molecular sequence information.

Citing MMDB