1FJE: SOLUTION STRUCTURE OF NUCLEOLIN RBD12 IN COMPLEX WITH SNRE RNA

Citation:
Abstract
The structure of the 28 kDa complex of the first two RNA binding domains (RBDs) of nucleolin (RBD12) with an RNA stem-loop that includes the nucleolin recognition element UCCCGA in the loop was determined by NMR spectroscopy. The structure of nucleolin RBD12 with the nucleolin recognition element (NRE) reveals that the two RBDs bind on opposite sides of the RNA loop, forming a molecular clamp that brings the 5' and 3' ends of the recognition sequence close together and stabilizing the stem-loop. The specific interactions observed in the structure explain the sequence specificity for the NRE sequence. Binding studies of mutant proteins and analysis of conserved residues support the proposed interactions. The mode of interaction of the protein with the RNA and the location of the putative NRE sites suggest that nucleolin may function as an RNA chaperone to prevent improper folding of the nascent pre-rRNA.
PDB ID: 1FJEDownload
MMDB ID: 14940
PDB Deposition Date: 2000/8/8
Updated in MMDB: 2007/11
Experimental Method:
solution nmr
Source Organism:
synthetic construct
Similar Structures:
Molecular Components in 1FJE
Label Count Molecule
Protein (1 molecule)
1
Nucleolin Rbd12
Molecule annotation
Nucleotide(1 molecule)
1
Snre RNA
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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