1F2X: STRUCTURE OF THE SINGLE-DOMAIN CAMELID ANTIBODY CAB-CA05

Citation:
Abstract
Grafting the antigen-binding loops onto a human antibody scaffold is a widely used technique to humanise murine antibodies. The success of this approach depends largely on the observation that the antigen-binding loops adopt only a limited number of canonical structures. Identification of the correct canonical structure is therefore essential. Algorithms that predict the main-chain conformation of the hypervariable loops using only the amino acid sequence often provide this information. Here, we describe new canonical loop conformations for the hypervariable regions H1 and H2 as found in single-domain antibody fragments of dromedaries or llama. Although the occurrence of these new loop conformations was not predicted by the algorithms used, it seems that they could occur in human or mouse antigen-binding loops. Their discovery indicates that the currently used set of canonical structures is incomplete and that the prediction algorithms should be extended to include these new structures.
PDB ID: 1F2XDownload
MMDB ID: 13692
PDB Deposition Date: 2000/5/30
Updated in MMDB: 2007/10
Experimental Method:
x-ray diffraction
Resolution: 2.1  Å
Source Organism:
Similar Structures:
Biological Unit for 1F2X: monomeric; determined by author
Molecular Components in 1F2X
Label Count Molecule
Protein (1 molecule)
1
Antibody Heavy Chain
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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