1F2J: CRYSTAL STRUCTURE ANALYSIS OF ALDOLASE FROM T. BRUCEI

Citation:
Abstract
Trypanosomatids, unicellular organisms responsible for several global diseases, contain unique organelles called glycosomes in which the first seven glycolytic enzymes are sequestered. We report the crystal structures of glycosomal fructose-1,6-bisphosphate aldolase from two major tropical pathogens, Trypanosoma brucei and Leishmania mexicana, the causative agents of African sleeping sickness and one form of leishmaniasis, respectively. Unlike mammalian aldolases, the T. brucei and L. mexicana aldolases contain nonameric N-terminal type 2 peroxisomal targeting signals (PTS2s) to direct their import into the glycosome. In both tetrameric trypanosomatid aldolases, the PTS2s from two different subunits form two closely intertwined structures. These "PTS2 dimers", which have very similar conformations in the two aldolase structures, are the first reported conformations of a glycosomal or peroxisomal PTS2, and provide opportunities for the design of trypanocidal compounds.
PDB ID: 1F2JDownload
MMDB ID: 14936
PDB Deposition Date: 2000/5/25
Updated in MMDB: 2000/12
Experimental Method:
x-ray diffraction
Resolution: 1.9  Å
Source Organism:
Similar Structures:
Biological Unit for 1F2J: tetrameric; determined by author and by software (PISA)
Molecular Components in 1F2J
Label Count Molecule
Proteins (4 molecules)
4
Fructose-bisphosphate Aldolase, Glycosomal
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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