1DZ8: Oxygen Complex Of P450cam From Pseudomonas Putida

Citation:
Abstract
Members of the cytochrome P450 superfamily catalyze the addition of molecular oxygen to nonactivated hydrocarbons at physiological temperature-a reaction that requires high temperature to proceed in the absence of a catalyst. Structures were obtained for three intermediates in the hydroxylation reaction of camphor by P450cam with trapping techniques and cryocrystallography. The structure of the ferrous dioxygen adduct of P450cam was determined with 0.91 angstrom wavelength x-rays; irradiation with 1.5 angstrom x-rays results in breakdown of the dioxygen molecule to an intermediate that would be consistent with an oxyferryl species. The structures show conformational changes in several important residues and reveal a network of bound water molecules that may provide the protons needed for the reaction.
PDB ID: 1DZ8Download
MMDB ID: 12899
PDB Deposition Date: 2000/2/18
Updated in MMDB: 2007/10
Experimental Method:
x-ray diffraction
Resolution: 1.9  Å
Source Organism:
Similar Structures:
Biological Unit for 1DZ8: monomeric; determined by software (PQS)
Molecular Components in 1DZ8
Label Count Molecule
Protein (1 molecule)
1
Cytochrome P450-cam
Molecule annotation
Chemicals (7 molecules)
1
2
2
1
3
2
4
1
5
1
* Click molecule labels to explore molecular sequence information.

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