1DW9: Structure Of Cyanase Reveals That A Novel Dimeric And Decameric Arrangement Of Subunits Is Required For Formation Of The Enzyme Active Site

Citation:
Abstract
BACKGROUND: Cyanase is an enzyme found in bacteria and plants that catalyzes the reaction of cyanate with bicarbonate to produce ammonia and carbon dioxide. In Escherichia coli, cyanase is induced from the cyn operon in response to extracellular cyanate. The enzyme is functionally active as a homodecamer of 17 kDa subunits, and displays half-site binding of substrates or substrate analogs. The enzyme shows no significant amino acid sequence homology with other proteins. RESULTS: We have determined the crystal structure of cyanase at 1.65 A resolution using the multiwavelength anomalous diffraction (MAD) method. Cyanase crystals are triclinic and contain one homodecamer in the asymmetric unit. Selenomethionine-labeled protein offers 40 selenium atoms for use in phasing. Structures of cyanase with bound chloride or oxalate anions, inhibitors of the enzyme, allowed identification of the active site. CONCLUSIONS: The cyanase monomer is composed of two domains. The N-terminal domain shows structural similarity to the DNA-binding alpha-helix bundle motif. The C-terminal domain has an 'open fold' with no structural homology to other proteins. The subunits of cyanase are arranged in a novel manner both at the dimer and decamer level. The dimer structure reveals the C-terminal domains to be intertwined, and the decamer is formed by a pentamer of these dimers. The active site of the enzyme is located between dimers and is comprised of residues from four adjacent subunits of the homodecamer. The structural data allow a conceivable reaction mechanism to be proposed.
PDB ID: 1DW9Download
MMDB ID: 23689
PDB Deposition Date: 1999/12/3
Updated in MMDB: 2007/10
Experimental Method:
x-ray diffraction
Resolution: 1.65  Å
Similar Structures:
Biological Unit for 1DW9: decameric; determined by software (PISA)
Molecular Components in 1DW9
Label Count Molecule
Proteins (10 molecules)
10
Cyanate Lyase(Gene symbol: cynS)
Molecule annotation
Chemicals (33 molecules)
1
10
2
23
* Click molecule labels to explore molecular sequence information.

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