1DU6: SOLUTION STRUCTURE OF THE TRUNCATED PBX HOMEODOMAIN

Citation:
Abstract
PBX is a member of the three amino acid loop extension (TALE) class of homeodomains. PBX binds DNA cooperatively with HOX homeodomain proteins that contain a conserved YPWM motif. The amino acids immediately C-terminal to the PBX homeodomain increase the affinity of the homeodomain for its DNA site and HOX proteins. We have determined the structure of the free PBX homeodomain using NMR spectroscopy. Both the PBX homeodomain and the extended PBX homeodomain make identical contacts with a 5'-TGAT-3' DNA site and a YPWM peptide. A fourth alpha-helix, which forms upon binding to DNA, stabilizes the extended PBX structure. Variations in DNA sequence selectivity of heterodimeric PBX-HOX complexes depend on the HOX partner; however, a comparison of five different HOX-derived YPWM peptides showed that each bound to PBX in the same way, differing only in the strength of the association.
PDB ID: 1DU6Download
MMDB ID: 14150
PDB Deposition Date: 2000/1/14
Updated in MMDB: 2007/11
Experimental Method:
solution nmr
Source Organism:
Similar Structures:
Molecular Components in 1DU6
Label Count Molecule
Protein (1 molecule)
1
Homeobox Protein Pbx1(Gene symbol: Pbx1)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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