1DSZ: Structure Of The RxrRAR DNA-Binding Domain Heterodimer In Complex With The Retinoic Acid Response Element Dr1

Citation:
Abstract
The 9-cis retinoic acid receptor (retinoid X receptor, RXR) forms heterodimers with the all-trans retinoic acid receptor (RAR) and other nuclear receptors on DNA regulatory sites composed of tandem binding elements. We describe the 1.70 A resolution structure of the ternary complex of RXR and RAR DNA-binding regions in complex with the retinoic acid response element DR1. The receptors recognize identical half-sites through extensive base-specific contacts; however, RXR binds exclusively to the 3' site to form an asymmetric complex with the reverse polarity of other RXR heterodimers. The subunits associate in a strictly DNA-dependent manner using the T-box of RXR and the Zn-II region of RAR, both of which are reshaped in forming the complex. The protein-DNA contacts, the dimerization interface and the DNA curvature in the RXR-RAR complex are distinct from those of the RXR homodimer, which also binds DR1. Together, these structures illustrate how the nuclear receptor superfamily exploits conformational flexibility and locally induced structures to generate combinatorial transcription factors.
PDB ID: 1DSZDownload
MMDB ID: 71895
PDB Deposition Date: 2000/1/10
Updated in MMDB: 2011/06
Experimental Method:
x-ray diffraction
Resolution: 1.7  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 1DSZ: tetrameric; determined by author
Molecular Components in 1DSZ
Label Count Molecule
Proteins (2 molecules)
1
Retinoic Acid Receptor Alpha(Gene symbol: RARA)
Molecule annotation
1
Retinoic Acid Receptor Rxr-alpha(Gene symbol: RXRA)
Molecule annotation
Nucleotides(2 molecules)
1
DNA (5'- D(*cp*ap*gp*gp*tp*cp*ap*ap*ap*gp*gp*tp*cp*ap*g)-3')
Molecule annotation
1
DNA (5'- D(*cp*tp*gp*ap*cp*cp*tp*tp*tp*gp*ap*cp*cp*tp*g)-3')
Molecule annotation
Chemicals (4 molecules)
1
4
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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