1DFU: Crystal Structure Of E.Coli Ribosomal Protein L25 Complexed With A 5s Rrna Fragment At 1.8 A Resolution

Citation:
Abstract
The crystal structure of Escherichia coli ribosomal protein L25 bound to an 18-base pair portion of 5S ribosomal RNA, which contains "loop E," has been determined at 1.8-A resolution. The protein primarily recognizes a unique RNA shape, although five side chains make direct or water-mediated interactions with bases. Three beta-strands lie in the widened minor groove of loop E formed by noncanonical base pairs and cross-strand purine stacks, and an alpha-helix interacts in an adjacent widened major groove. The structure of loop E is largely the same as that of uncomplexed RNA (rms deviation of 0.4 A for 11 base pairs), and 3 Mg(2+) ions that stabilize the noncanonical base pairs lie in the same or similar locations in both structures. Perhaps surprisingly, those residues interacting with the RNA backbone are the most conserved among known L25 sequences, whereas those interacting with the bases are not.
PDB ID: 1DFUDownload
MMDB ID: 71860
PDB Deposition Date: 1999/11/21
Updated in MMDB: 2011/06
Experimental Method:
x-ray diffraction
Resolution: 1.8  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 1DFU: trimeric; determined by author
Molecular Components in 1DFU
Label Count Molecule
Protein (1 molecule)
1
Ribosomal Protein L25(Gene symbol: rplY)
Molecule annotation
Nucleotides(2 molecules)
1
5S rRNA
Molecule annotation
1
5S rRNA
Molecule annotation
Chemicals (5 molecules)
1
5
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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