1DEV: CRYSTAL STRUCTURE OF SMAD2 MH2 DOMAIN BOUND TO THE SMAD-BINDING DOMAIN OF SARA

Citation:
Abstract
The Smad proteins mediate transforming growth factor-beta (TGFbeta) signaling from the transmembrane serine-threonine receptor kinases to the nucleus. The Smad anchor for receptor activation (SARA) recruits Smad2 to the TGFbeta receptors for phosphorylation. The crystal structure of a Smad2 MH2 domain in complex with the Smad-binding domain (SBD) of SARA has been determined at 2.2 angstrom resolution. SARA SBD, in an extended conformation comprising a rigid coil, an alpha helix, and a beta strand, interacts with the beta sheet and the three-helix bundle of Smad2. Recognition between the SARA rigid coil and the Smad2 beta sheet is essential for specificity, whereas interactions between the SARA beta strand and the Smad2 three-helix bundle contribute significantly to binding affinity. Comparison of the structures between Smad2 and a comediator Smad suggests a model for how receptor-regulated Smads are recognized by the type I receptors.
PDB ID: 1DEVDownload
MMDB ID: 12368
PDB Deposition Date: 1999/11/15
Updated in MMDB: 2007/10
Experimental Method:
x-ray diffraction
Resolution: 2.2  Å
Source Organism:
Similar Structures:
Biological Unit for 1DEV: dimeric; determined by author and by software (PISA)
Molecular Components in 1DEV
Label Count Molecule
Proteins (2 molecules)
1
MAD (Mothers Against Decapentaplegic, Drosophila) Homolog 2(Gene symbol: SMAD2)
Molecule annotation
1
Smad Anchor for Receptor Activation(Gene symbol: ZFYVE9)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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