1D9N: SOLUTION STRUCTURE OF THE METHYL-CPG-BINDING DOMAIN OF THE METHYLATION-DEPENDENT TRANSCRIPTIONAL REPRESSOR MBD1/PCM1

Citation:
Abstract
CpG methylation in vertebrates is important for gene silencing, alterations in chromatin structure and genomic stability, and differences in the DNA-methylation status are correlated with imprinting phenomena, carcinogenesis and embryonic development. Methylation signals are interpreted by protein factors that contain shared methyl-CpG-binding domains (MBDs). We have determined the solution structure of the MBD of the human methylation-dependent transcriptional repressor MBD1 by multi-dimensional heteronuclear NMR spectroscopy. It folds into an alpha/beta-sandwich structure with characteristic loops. Basic residues conserved in the MBD family are largely confined to one face of this fold and a flexible loop, which together form a large positively charged surface. Site-directed mutagenesis and chemical shift changes upon complexing with a methylated DNA facilitated identification of this surface as the DNA interaction site. In addition to three basic residues, conserved Tyr34 and Asp32 were shown to be important for the DNA binding.
PDB ID: 1D9NDownload
MMDB ID: 14755
PDB Deposition Date: 1999/10/28
Updated in MMDB: 2000/12
Experimental Method:
solution nmr
Source Organism:
Similar Structures:
Molecular Components in 1D9N
Label Count Molecule
Protein (1 molecule)
1
Methyl-cpg-binding Protein Mbd1(Gene symbol: MBD1)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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