1CX6: T4 LYSOZYME SUBSTITUTED WITH SELENOMETHIONINE

Citation:
Abstract
The availability of a series of phage T4 lysozymes with up to 14 methionine residues incorporated within the protein has made it possible to systematically compare the effect on protein stability of selenomethionine relative to methionine. Wild-type lysozyme contains two fully buried methionine residues plus three more on the surface. The substitution of these methionine residues with selenomethionine slightly stabilizes the protein. As more and more methionine residues are substituted into the protein, there is a progressive loss of stability. This is, however, increasingly offset in the selenomethionine variants, ultimately resulting in a differential increase in melting temperature of about 7 degrees C. This increase, corresponding to about 0.25 kcal/mol per substitution, is in reasonable agreement with the difference in the solvent transfer free energy between the two amino acids.
PDB ID: 1CX6Download
MMDB ID: 11746
PDB Deposition Date: 1999/8/28
Updated in MMDB: 2007/10
Experimental Method:
x-ray diffraction
Resolution: 2.01  Å
Source Organism:
Similar Structures:
Biological Unit for 1CX6: monomeric; determined by author
Molecular Components in 1CX6
Label Count Molecule
Protein (1 molecule)
1
Lysozyme(Gene symbol: e)
Molecule annotation
Chemicals (3 molecules)
1
2
2
1
* Click molecule labels to explore molecular sequence information.

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